°æÈñ´ëÇб³ ½Ä¹°±â´ÉüÀ¯ÀüÇÐ ½ÇÇè½Ç

»çÀÌÆ® ¸Ê ã¾Æ¿À½Ã´Â ±æ ¿¬¶ôó ¾È³»

 

±³¼ö´Ô ÇÁ·ÎÇÊ ¿¬±¸ºÐ¾ß ¼Ò°³ ¹ßÇ¥³í¹® ½ÇÇè½Ç ±¸¼º¿ø °Ô½ÃÆÇ °ü·Ã»çÀÌÆ®

 

 

> °Ô½ÃÆÇ Àüüº¸±â > ¾Ë¸²»çÇ×


View Article
Name
  ÀüÁ¾¼º 2006-04-03 09:55:52 | Hit : 13834 | Vote : 2740
Subject   ½Ä¹°´ë»ç/»ý¸í°øÇпø ¼¼¹Ì³ª
2006³â 4¿ù 6ÀÏ ¿ÀÈÄ 4½Ã 30ºÐ
°­Èƽ (Àü³²´ë)
Á¶¸¸È£ (°æÈñ´ë)



The Roles of RNA-Binding Proteins in Plant Stress Responses

Hunseung Kang (°­ÈƽÂ)
Department of Plant Biotechnology, Chonnam National University
(Àü³²´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ ½Ä¹°»ý¸í°øÇкÎ)

Although a number of studies have demonstrated that RNA-binding proteins (RBPs) are involved in plant responses to environmental stresses, the functional roles of RBPs under stress conditions are largely unknown. To understand the function of RBPs and the mechanism by which RBP-mediated posttranscriptional regulation of gene expression in plants under various environmental stresses, the gene families encoding glycine rich-RBP (GR-RBP), zinc finger-RBP, and cold shock domain-RBP (CSD-RBP) were characterized for their nucleic acid-binding property, expression pattern and function in stress responses in Arabidopsis thaliana. Nucleic acid-binding capabilities of the RBPs vary depending on the types of RBPs. All the RBPs investigated were highly up regulated by cold stress, and were differently regulated by drought or high salinity stress. Analyses of the T-DNA tagged knockout mutants and overexpression transgenic plants revealed that RBPs play fundamental roles in cold, salt, or drought stress adaptation process in plant. Freezing test and heterologous expression of RBPs in Escherichia coli revealed that RBPs contribute to freezing tolerance in Arabidopsis and help E. coli grow and survive better during cold shock. Two-dimensional gel and proteome analyses suggest several target genes modulated by each RBPs. These results reveal that GR-RBPs, CSD-RBPs, and zinc finger-RBPs have multiple functions in plant response to various stresses, and particularly play roles as RNA chaperones during cold or freezing adaptation process. The current findings, limitations, and future prospects of RNA-binding proteins in plant responses to diverse environmental stimuli will also be discussed.


Lignan Biosynthesis in Larrea tridentata: Enantiospecific Aromatic Hydroxylation of Larreatricin

°æÈñ´ë Á¶¸¸È£

Creosote bush (Larrea tridentata) was widely used in traditional medicine among indigenous people of America for digestive disorders, rheumatism, venereal diseases and sores.  Nordihydroguaiaretic acid (NDGA), the major constituent of its resinous exudate (5-10 % of leaf dry weight), is a powerful antioxidant and has been employed in non-food applications such as stabilizing polymer, rubber, perfumery oil and photographic formulations. Beside NDGA, creosote bush accumulates a vast array of 8-8¢ linked allyphenol-derived lignans. Of which, 3¢-O-methyl-NDGA and methylated/ acetylated NDGA derivatives has antiviral activities against HIV, herpes simplex virus and/or human papilloma virus.
Larreatricin, presumed coupling product of an allyphynol, anol, is considered to be an important intermediate involving regiospecific hydroxylation at C-3¢ in the biosynthesis of lignans in L. tridentata. A polyphenol oxidase catalyzing 3¢-hydroxylation of larreatricin to 3¢-hydroxylarreatricin was purified from the leaves of creosote bush by DEAE-Sepharose, Hydroxylapatite, and Phenyl Sepharose column chromatographies. The enzyme has a molecular weight of ~43 kD, and a broad pH optimum of pH 6 - 8. The enzymatic reaction is enantiospecific, with larreatricin 3¢-hydroxylase selectively introducing a hydroxyl group into (+)-larreatricin but not the corresponding enantiomeric form, (-)-larreatricin.


No Subject Name Date Hit
32  Rice Functional Genomics Symposium 2006: Insertional Mutagenesis and Gene Mining   ÀüÁ¾¼º 2006/04/05 47869
 ½Ä¹°´ë»ç/»ý¸í°øÇпø ¼¼¹Ì³ª   ÀüÁ¾¼º 2006/04/03 13834
30  [WCU]Gurdev Khush ÀÎÅͺä 2010-05-26 ³»ÀϽŹ® Ä®·³   Ȩ¿î¿µÀÚ 2010/06/10 13058
29  [WCU] 2011³â OCW(Open Course Ware) ÇöȲ ¹× °­ÀÇ(Adavanced New-Plant-Type crop breeding) Æò°¡   Ȩ¿î¿µÀÚ 2011/05/12 12763
28  [WCU] Khush ¹Ú»ç´Ô Çѱ¹ ¹æ¹® ÀÏÁ¤ [3Â÷³âµµ ÁøÇàÁß]   Ȩ¿î¿µÀÚ 2011/05/12 11909
27  11¿ù 18ÀÏ Confocal »ç¿ë¹ý Ư°­   ÀüÁ¾¼º 2004/11/18 11797
26  [WCU] 2012³â OCW(Open Course Ware) ÇöȲ - ½Ä¹°ºÐÀÚ»ý¹°ÇРƯ·Ð   Ȩ¿î¿µÀÚ 2012/08/02 11775
25  [WCU] 2013³â OCW(Open Course Ware) ÇöȲ - ½Ä¹°ºÐÀÚÀ°Á¾ÇРƯ·Ð   ¼Û¹Î¿µ 2013/07/25 11743
24  [WCU] ÇöÀç, ±×¸®°í ¹Ì·¡ÀÇ ½Ä·®¹®Á¦ ÇØ°áÀ» À§ÇÑ ¿¬±¸»ý¸í°øÇаú À°Á¾ÀÇ À¶ÇÕ¿¬±¸ ¡®Ä£È¯°æ ´Ù¼öÈ® ½ÅÃÊÇü º­ °³¹ß¡¯ -Dynamic Korea 12¿ùÈ£   Ȩ¿î¿µÀÚ 2009/12/02 11405
23  [KH news- Creation] ±âȹ-½ÅÀÓ±³¼ö ¸±·¹ÀÌ ÀÎÅͺä, »ý¸í°øÇпø °ÅµªÄí½¬±³¼ö   Ȩ¿î¿µÀÚ 2010/07/28 11365
22   [WCU] Khush ¹Ú»ç´Ô Çѱ¹ ¹æ¹® ÀÏÁ¤ [4Â÷³âµµ ÁøÇàÁß]   Ȩ¿î¿µÀÚ 2012/04/19 11232
21  ´ëÇпø Çаú ½Å¼³ [Plant Molecular Systems Biotechnology Çаú]   Ȩ¿î¿µÀÚ 2009/10/13 11198
20  [WCU] ½ºÆ÷Ã÷µ¿¾Æ ½ÅÁö½ÄÀδë»ó °æÈñ´ëÇб³ »ý¸í°øÇпø ÀüÁ¾¼º ±³¼ö   Ȩ¿î¿µÀÚ 2011/03/10 11169
19  [WCU] [»ç¶÷µé] ¼¼°è º­ À°Á¾ ´ëºÎ °Åµª Äí½¬ ¹Ú»ç -Áß¾ÓÀϺ¸ 7¿ù 9ÀÏÀÚ   Ȩ¿î¿µÀÚ 2009/09/20 10186
18  [WCU] 2009³â OCW(Open Course Ware) ÇöȲ¹× ¹ÙÀÌ¿À¿¡³ÊÁö °­ÀÇÆò°¡   Ȩ¿î¿µÀÚ 2009/10/14 10120
17  [WCU] »ç¾÷¼Ò°³   Ȩ¿î¿µÀÚ 2009/07/21 9961
16  [WCU] Dr. Gurdev S. Khush ¼Ò°³   Ȩ¿î¿µÀÚ 2009/08/10 9851
15  ½Ä¹°´ë»ç¿¬±¸¼¾ÅÍ µ¿°èÄÝ·ÎÄû¿ò   ÀüÁ¾¼º 2005/12/05 9726
14  2005³â ¸ð³»±â (1Â÷)   ÀüÁ¾¼º 2005/05/19 9626
13  Çѱ¹±¤°úÇÐȸ   ÀüÁ¾¼º 2006/02/21 9626
  1 [2] 
Copyright 1999-2024 Zeroboard / skin by daerew

 

Copyright 2004 (c) Plant Functional Genomics Lab. Department of Biological Sciences, Kyung Hee University
1, Sochen-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea. Zip code : 446-701 Tel : 82-31-201-3671
All rights reserved. The last homepage modified 10/22/2004 09:41:15.
- Counting status : visited today, visited yesterday, visited in sum.