경희대학교 식물기능유전체학 실험실

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AUTHOR

Young-Su Seo, Maria R. Rojas, Jung-Youn Lee, Sang-Won Lee, Jong-Seong Jeon, Pamela Ronald, William J. Lucas, and Robert L. Gilbertson (2006)

TITLE

A viral resistance gene from common bean functions across plant families and is upregulated in a non-virus-specific manner

JOURNAL

Proc Natl Acad Sci USA 103: 11856-11861

File

RT4_4.pdf (1.65 MB)

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The Abstract Of The Paper

Genes involved in a viral resistance response in common bean (Phaseolus vulgaris cv.
Othello) were identified by inoculating a geminivirus reporter (Bean dwarf mosaic
virus expressing the green fluorescent protein), extracting RNA from tissue
undergoing the defense response, and amplifying sequences with degenerate R gene
primers. One such gene (a TIR-NBS-LRR gene, RT4-4) was selected for functional
analysis in which transgenic Nicotiana benthamiana were generated and screened
for resistance to a range of viruses. This analysis revealed that RT4-4 did not confer
resistance to the reporter geminivirus; however, it did activate a resistance-related
response (systemic necrosis) to 7 strains of Cucumber mosaic virus (CMV) from
pepper or tomato, but not to a CMV strain from common bean. Of these 8 CMV
strains, only the strain from common bean systemically infected common bean cv.
Othello. Additional evidence that RT4-4 is a CMV R gene came from the detection
of resistance response markers in CMV-challenged leaves of RT4-4 transgenic
plants, and the identification of the CMV 2a gene product as the elicitor of the
necrosis response. These findings indicate that RT4-4 functions across two plant
families and is upregulated in a non-virus-specific manner. This experimental
approach holds promise for providing insights into the mechanisms by which plants
activate resistance responses against pathogens.

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