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View Article
AUTHOR
  Phee B-K, Cho J-H, Pack S, Jung J-H, Lee Y-H, Jeon J-S, Bhoo S-H, Hahn T-R. (2004)
TITLE   Proteomic analysis of the response of Arabidopsis chloroplast proteins to high light stress.
JOURNAL   Proteomics. 4:3560-3568
File
   Phee_et_al.pdf (125.5 KB) Download : 643
  The Abstract Of The Paper
Light is an essential environmental factor in the progression of plant growth and development but prolonged exposure to high levels of light stress can cause cellular damage and ultimately result in the death of the plant.  Plants can respond defensively to this stress for a limited period and this involves changes to their gene expression profiles.  Proteomic approaches were therefore applied to the study of the response to high light stress in the Arabidopsis thaliana plant species.  Wild type Arabidopsis was grown under normal light (100 ¥ìmol photons•m-2•s-1) conditions and then subjected to high light (1,000 ¥ìmol photons•m-2•s-1) stress.  Chloroplasts were then isolated from these plants and both soluble and insoluble proteins were extracted and subjected to 2-D gel electrophoresis.  The resolved proteins were subsequently identified by MALDI-TOF mass spectrometry (MS) and comparative database analysis.  64 protein spots, that were identified as candidate factors that responded to high light stress, were then selected for analysis and 52 of these were successfully identified using MALDI-TOF MS analysis.  35 of the 52 identified proteins were found to decrease their expression levels during high light stress and a further 14 of the candidate proteins had upregulated expression levels under these conditions.  Most of the proteins that were downregulated during high light stress are involved in photosynthesis pathways.  However, many of the 14 upregulated proteins were identified as previously well known high light stress-related proteins such as heat shock proteins (HSP), dehydroascorbate reductase (DHAR) and superoxide dismutase (SOD).  Three novel proteins that were more highly expressed during periods of high light stress, but had no clear functional relationship to these conditions, were also identified in this study.
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