경희대학교 식물기능유전체학 실험실

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AUTHOR

Kim SR, Jeon JS, An G (2011)

TITLE

Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice

JOURNAL

Methods Mol Biol 678:139-146 (Jan)

File

iPCR.pdf (1.51 MB)

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The Abstract Of The Paper

The central goal of current genomics research in plants, as in other organisms, is to elucidate the functions of every gene. Insertional mutagenesis using known DNA sequences such as T-DNA is a powerful tool in functional genomics. Development of efficient methods for isolating the genomic sequences flanking insertion elements accelerates the systematic cataloging of insertional mutants, and thus allows functions to be assigned to uncharacterized genes via reverse genetic approaches. In our current study, we report a rapid and efficient inverse PCR (iPCR) method for the isolation of gene tags in T-DNA mutant lines of rice (Oryza sativa), a model monocot plant.

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