경희대학교 식물기능유전체학 실험실

View Article

AUTHOR

Chang-Hyo Goh, Kouji Satoh, Shoshi Kikuchi, Seong-Cheol Kim, Suk-Min Ko, Hong-Gyu Kang, Jong-Seong Jeon, Cheol Soo Kim, Youn-Il Park (2010)

TITLE

Mitochondrial activity in illuminated leaves of chlorophyll-deficient mutant rice (OsCHLH)

JOURNAL

Plant Biotechnology Reports 4:281–291 (2010.10)

File

OsCHLH.pdf (481.1 KB)

Download : 430

The Abstract Of The Paper

The rice CHLH gene encodes the Mg2+-chelatase H subunit, which is involved in chlorophyll biosynthesis. Growth of the chlorophyll-deficient OsCHLH mutant is supported by mitochondrial activity. In this study, we investigated the activity of mitochondrial respiration in the illuminated leaves during OsCHLH seedling development. Growth of mutant plants was enhanced in the presence of 3% sucrose, which may be used by mitochondria to meet cellular energy requirements. ATP content in these mutants was however significantly lowered in light conditions. Low cytosolic levels of NADH in illuminated OsCHLH mutant leaves further indicated the inhibition of mitochondrial metabolism.
This down regulation was particularly evident for oxidative-stress-responsive genes in the mutant under light conditions. Hydrogen peroxide levels were higher in OsCHLH mutant leaves than in wildtype leaves; this increase was largely caused by the impairment of the expression of the antioxidant genes, such as OsAPX1, OsRAC1, and OsAOXc in knockout plants. Moreover, treatment of mesophyll protoplasts with ascorbic acid or catalase recovered ATP content in the mutants. Taken together, these results suggest that the light-mediated inhibition of mitochondrial activity leads to the stunted growth of CHLH rice seedlings.

Prev	벼멸구 저항성 벼 품종을 선별하기위한 마커	홈운영자	2010/10/06

Next	Rice functional genomics using T-DNA mutants 2010.06	전종성	2010/07/20