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View Article
AUTHOR
  Hye-Min Lim, Jung-Il Cho, Sichul Lee, Man-Ho Cho, Seong Hee Bhoo, Gynheung An, Tae-Ryong Hahn, Jong-Seong Jeon (2007)
TITLE   Identification of a 20-bp regulatory element of the Arabidopsis pyrophosphate:fructose-6-phosphate 1-phosphotransferase ¥á2 gene that is essential for expression
JOURNAL   Plant Cell Reports 26: 683-692
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  The Abstract Of The Paper
Arabidopsis harbors two  and two  genes of pyrophosphate:fructose-6-phosphate 1-phosphotransferase (PFP). The spatial expression patterns of the two AtPFP genes were analyzed using transgenic plants containing a promoter:: ©¬-glucuronidase (GUS) fusion construct. Whereas the AtPFP1 promoter was found to be ubiquitously active in all tissues, the AtPFP2 promoter is preferentially expressed in specific heterotrophic regions of the Arabidopsis plant such as the trichomes of leaves, cotyledon veins, roots, and the stamen and gynoecium of the flowers. Serial deletion analysis of the AtPFP2 promoter identified a key regulatory element from nucleotides -194 to -175, CGAAAAAGGTAAGGGTATAT, which we have termed PFP2 and which is essential for AtPFP2 gene expression. Using a GUS fusion construct driven by this 20-bp sequence in conjunction with a -46 CaMV35S minimal promoter, we also demonstrate that PFP2 is sufficient for normal AtPFP2 expression. Hence, this element can not only be used to isolate essential DNA-binding protein(s) that control the expression of the carbon metabolic enzyme AtPFP2, but has also the potential to be utilized in the production of useful compounds in a specific organ such as the leaf trichomes.
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