°æÈñ´ëÇб³ ½Ä¹°±â´ÉüÀ¯ÀüÇÐ ½ÇÇè½Ç

»çÀÌÆ® ¸Ê ã¾Æ¿À½Ã´Â ±æ ¿¬¶ôó ¾È³»

 

±³¼ö´Ô ÇÁ·ÎÇÊ ¿¬±¸ºÐ¾ß ¼Ò°³ ¹ßÇ¥³í¹® ½ÇÇè½Ç ±¸¼º¿ø °Ô½ÃÆÇ °ü·Ã»çÀÌÆ®

 

 

> ¹ßÇ¥³í¹®


View Article
AUTHOR
  Yi G, Lee S-K, Hong Y-K, Cho Y-C, Nam M-H, Kim S-C, Han S-S, Wang G-L, Hahn T-R, Ronald PC, Jeon J-S. (2004)
TITLE   Use of Pi5(t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea.
JOURNAL   Theor Appl Genetics 109: 978-985
File
   Pi5_MAS.pdf (917.7 KB) Download : 756
  The Abstract Of The Paper
Identification of the PCR markers tightly linked to genes that encode important agronomic traits is useful for marker-assisted selection (MAS). The rice Pi5(t) locus confers broad-spectrum resistance to Magnaporthe grisea, the causal agent of rice blast disease. It has been hypothesized that the Pi5(t) locus carries the same gene as that encoded by the Pi3(t) and Pii(t) loci. We developed three PCR-based dominant markers (JJ80-T3, JJ81-T3, and JJ113-T3) from three previously identified BIBAC clones—JJ80, JJ81, and JJ113—that are linked to the Pi5(t) locus. PCR analysis of 24 monogenic lines revealed that these markers are present only in lines that carry Pi5(t), Pi3(t), and Pii(t). PCR and DNA gel-blot analysis of candidate resistance lines using JJ80-T3, JJ81-T3, and JJ113-T3 indicated that Tetep is the likely donor of Pi5(t). Of the 184 rice varieties tested, 34 carried the JJ80-T3-, JJ81-T3-, and JJ113-T3-specific bands. Disease evaluation of those 34 varieties revealed that all conferred resistance to PO6-6. The genomic structure of three of these resistant varieties (i.e., IR72, Taebaeg, Jahyangdo) is most similar to that of Pi5(t). Our results demonstrate the usefulness of the JJ80-T3, JJ81-T3, and JJ113-T3 markers for MAS for M. grisea resistance.
 Prev   Proteomic analysis of the response of Arabidopsis chloroplast proteins to high light stress.
  2004/09/20 
 Next   Analysis of Carbon Metabolism in Transgenic Arabidopsis Thaliana Transformed with the Cyanobacterial Sucrose Phosphate Synthase Gene.
  1970/01/01 


Copyright 1999-2024 Zeroboard / skin by daerew

 

Copyright 2004 (c) Plant Functional Genomics Lab. Department of Biological Sciences, Kyung Hee University
1, Sochen-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea. Zip code : 446-701 Tel : 82-31-201-3671
All rights reserved. The last homepage modified 10/22/2004 09:41:15.
- Counting status : visited today, visited yesterday, visited in sum.