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Young-Su Seo, Maria R. Rojas, Jung-Youn Lee, Sang-Won Lee, Jong-Seong Jeon, Pamela Ronald, William J. Lucas, and Robert L. Gilbertson (2006) |
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A viral resistance gene from common bean functions across plant families and is upregulated in a non-virus-specific manner |
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JOURNAL |
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Proc Natl Acad Sci USA 103: 11856-11861 |
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The Abstract Of The Paper |
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Genes involved in a viral resistance response in common bean (Phaseolus vulgaris cv.
Othello) were identified by inoculating a geminivirus reporter (Bean dwarf mosaic
virus expressing the green fluorescent protein), extracting RNA from tissue
undergoing the defense response, and amplifying sequences with degenerate R gene
primers. One such gene (a TIR-NBS-LRR gene, RT4-4) was selected for functional
analysis in which transgenic Nicotiana benthamiana were generated and screened
for resistance to a range of viruses. This analysis revealed that RT4-4 did not confer
resistance to the reporter geminivirus; however, it did activate a resistance-related
response (systemic necrosis) to 7 strains of Cucumber mosaic virus (CMV) from
pepper or tomato, but not to a CMV strain from common bean. Of these 8 CMV
strains, only the strain from common bean systemically infected common bean cv.
Othello. Additional evidence that RT4-4 is a CMV R gene came from the detection
of resistance response markers in CMV-challenged leaves of RT4-4 transgenic
plants, and the identification of the CMV 2a gene product as the elicitor of the
necrosis response. These findings indicate that RT4-4 functions across two plant
families and is upregulated in a non-virus-specific manner. This experimental
approach holds promise for providing insights into the mechanisms by which plants
activate resistance responses against pathogens.
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Copyright 2004 (c) Plant Functional Genomics Lab. Department of Biological Sciences, Kyung Hee University
1, Sochen-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Korea. Zip code : 446-701 Tel : 82-31-201-3671
All rights reserved. The last homepage modified 10/22/2004 09:41:15.
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